mouse anti-stip1  (Abnova)

Journal: Oncotarget

Article Title: Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

doi: 10.18632/oncotarget.15222

Figure Lengend Snippet: ( A ) After 24-h culture of the RCC tumor cells, secreted STIP1 in the culture medium supernatant was detected, while the intracellular protein GAPDH was not detected in the culture medium supernatant indicating no leakage of intracellular components into the culture media. ( B – C ). STIP1 was detected in the purified cell surface protein, while no trace of HSP90 was identified, while HSP90 was detected in the total cell lysates (C). ( D ) Quantification of STIP1 protein in the culture medium supernatant as secreted STIP1 (left panel), and in the purified cell surface protein as outer cell surface STIP1 (right panel). Experiments were triplicated, and mean ± SD was presented. *p < 0.05, vs OS-RC-2; # p < 0.05, vs ACHN. In all panels, western blot images have been cropped to show the protein of interest, and all blots were performed under the same experimental conditions.

Article Snippet: The formalin-fixed, paraffin-embedded tissue sections were stained with a primary mouse anti-human STIP1 monoclonal antibody (1:200, Boster Biotechnology, Wuhan, China) with the Ventana Basic DAB (3,3-diaminobenzidine) Detection kit (Boster Biotechnology, Wuhan, China).

Techniques: Purification, Western Blot

Journal: Oncotarget

Article Title: Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

doi: 10.18632/oncotarget.15222

Figure Lengend Snippet: ( A ) STIP1 protein was examined in 19 protein specimens from primary RCC tumors (P, n = 7) and bone metastatic samples (M, n = 12). Proteins were electrophoresed in two 10% SDS-PAGE gels, and were subsequently transferred to two PVDF membranes (10 and 9 specimens for gels 1and 2, respectively). ( B ) The intensity of STIP1 in each lane was normalized with the intensity of GAPDH. *p < 0.05. Experiments were duplicated, and western blot images shown have been cropped to show the protein of interest, and all blots were performed under the same experimental conditions. ( C ) Representative immunohistochemistry staining of STIP1 in primary RCC and bone metastasis tumors. Both intracellular and extracellular STIP1 immunoreactivity was examined as shown in the inset. Images were taken under 20× objective. Scale bar: 50 μm. ( D ) Correlation between the H scores of STIP1 in primary RCC and bone metastasis tumors of the 10 pairs of matched samples. R 2 = 0.6923.

Article Snippet: The formalin-fixed, paraffin-embedded tissue sections were stained with a primary mouse anti-human STIP1 monoclonal antibody (1:200, Boster Biotechnology, Wuhan, China) with the Ventana Basic DAB (3,3-diaminobenzidine) Detection kit (Boster Biotechnology, Wuhan, China).

Techniques: SDS Page, Western Blot, Immunohistochemistry, Staining

Journal: Oncotarget

Article Title: Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

doi: 10.18632/oncotarget.15222

Figure Lengend Snippet: ( A ) STIP1 mRNA expressed highly in the advanced stage RCC tumors. ( B ) STIP1 mRNA expressed highly in the high grades RCC tumors. ( C ) STIP1 mRNA expressed highly in the metastatic RCC tumors (M1+). Overexpression gene rank and P value were generated by the Oncomine algorithms. Fold change was log 2 based.

Article Snippet: The formalin-fixed, paraffin-embedded tissue sections were stained with a primary mouse anti-human STIP1 monoclonal antibody (1:200, Boster Biotechnology, Wuhan, China) with the Ventana Basic DAB (3,3-diaminobenzidine) Detection kit (Boster Biotechnology, Wuhan, China).

Techniques: Over Expression, Generated

Journal: Oncotarget

Article Title: Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

doi: 10.18632/oncotarget.15222

Figure Lengend Snippet: ( A ) Proliferation of OS-RC-BM5 cells under indicated treatment. ( B ) Cell cycle analysis of OS-RC-BM5 cells under indicated treatment. ( C ) shRNA knockdown of STIP1 in OS-RC-BM5 cells. ( D ) Representative images of Ki67 immunoreactivity in the bone metastasis tumors. Images were taken under 20× objective. Scale bar: 50 μm. ( E ) Correlation between the H scores of STIP1 and percentage of Ki67-positive cells in the same bone metastasis tumors ( n =). R 2 = 0.5868.

Article Snippet: The formalin-fixed, paraffin-embedded tissue sections were stained with a primary mouse anti-human STIP1 monoclonal antibody (1:200, Boster Biotechnology, Wuhan, China) with the Ventana Basic DAB (3,3-diaminobenzidine) Detection kit (Boster Biotechnology, Wuhan, China).

Techniques: Cell Cycle Assay, shRNA, Knockdown

Journal: Oncotarget

Article Title: Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

doi: 10.18632/oncotarget.15222

Figure Lengend Snippet: ( A ) Representative images of the Transwell membranes with tumor cells migrated to the counter side of the chamber. Note, cell migration was not affected when hrSTIP1 was added into the lower chamber (+ lower chamber). ( B ) Quantification of the migration analysis with three repeats. *p < 0.05, vs vehicle; # p < 0.05, vs hrSTIP1+anti-STIP1.

Article Snippet: The formalin-fixed, paraffin-embedded tissue sections were stained with a primary mouse anti-human STIP1 monoclonal antibody (1:200, Boster Biotechnology, Wuhan, China) with the Ventana Basic DAB (3,3-diaminobenzidine) Detection kit (Boster Biotechnology, Wuhan, China).

Techniques: Migration

Journal: PLoS ONE

Article Title: The Cytosolic Chaperonin CCT/TRiC and Cancer Cell Proliferation

doi: 10.1371/journal.pone.0060895

Figure Lengend Snippet: The amount of ( A ) Hop/p60, ( B ) PhLP3 and ( C ) PFD in 18 cancer and a normal (MRC5-SV2) cell lines extracts, a non-cancer liver homogenate (HNCL) and rabbit reticulocyte lysate (RRL) was determined by comparing the chemiluminescence signal recorded for Hop/p60, PhLP3 and PFD in cell extracts diluted 4 and 16 fold to that of known amounts of the proteins on the same Western blots.

Article Snippet: Mouse polyclonal antibodies against human PhLP3 (anti TXNDC9), Hop/p60 (anti STIP1) and PFD5 (anti PFDN5) were purchased from Abnova (Ontario, Canada).

Techniques: Western Blot

Journal: PLoS ONE

Article Title: The Cytosolic Chaperonin CCT/TRiC and Cancer Cell Proliferation

doi: 10.1371/journal.pone.0060895

Figure Lengend Snippet: CCT/TRiC folding activity in ( A ) MIA-PaCa-2 and ( C ) OVCAR-8 cell extracts before and after CCT/TriC, Hop/p60 or PhLP3 immunodepletion was assayed using [ 35 S]-labeled, denatured β-actin. The protein concentration of the MIA-PaCa-2 and OVCAR-8 cell extracts was 2.5 and 15 mg/ml, respectively. Native β-actin band intensity was measured using a PhosphorImager. The amount of native β-actin measured after immunodepletion is expressed as a fraction of the amount measured for the cell extracts before immunodepletion in ( B ) MIA-PaCa-2 and ( D ) OVCAR-8 cell extracts.

Article Snippet: Mouse polyclonal antibodies against human PhLP3 (anti TXNDC9), Hop/p60 (anti STIP1) and PFD5 (anti PFDN5) were purchased from Abnova (Ontario, Canada).

Techniques: Activity Assay, Immunodepletion, Labeling, Protein Concentration